Session 1: Empowering Drug Metabolite Identification with a Novel LC/MS Software Workflow

Identifying drug metabolites is crucial for understanding drug behavior, efficacy, and safety, but it poses significant challenges due to the complexity and variability of biotransformation products. In this webinar, we describe a novel hi-res LC/MS strategy for drug metabolite identification and demonstrate with several known drugs. This approach combines cutting-edge software to predict drug biotransformations, search MS/MS data against these structures using molecular fingerprinting, and map identifications to molecular features from differential analysis of drug-treated liver microsomes. The workflow addresses the challenge of identifying drug biotransformation products that are often absent from known spectral libraries.