FOR IMMEDIATE RELEASE
ACS News Service Weekly PressPac: October 13, 2021
Warm milk makes you sleepy — peptides could explain why
“Identification and Screening of Potential Bioactive Peptides with Sleep-Enhancing Effects in Bovine Milk Casein Hydrolysate”
Journal of Agricultural and Food Chemistry
According to time-honored advice, drinking a glass of warm milk at bedtime will encourage a good night’s rest. Milk’s sleep-enhancing properties are commonly ascribed to tryptophan, but scientists have also discovered a mixture of milk peptides, called casein tryptic hydrolysate (CTH), that relieves stress and enhances sleep. Now, researchers reporting in ACS’ Journal of Agricultural and Food Chemistry have identified specific peptides in CTH that might someday be used in new, natural sleep remedies.
According to the U.S. Centers for Disease Control and Prevention, one-third of U.S. adults don’t get enough sleep. Sedatives, such as benzodiazepines and zolpidem, are commonly prescribed for insomnia, but they can cause side effects, and people can become addicted to them. Many sedatives work by activating the GABA receptor, a protein in the brain that suppresses nerve signaling. Scientists have also discovered several natural peptides, or small pieces of proteins, that bind the GABA receptor and have anti-anxiety and sleep-enhancing effects. For example, treating a protein in cow’s milk, called casein, with the digestive enzyme trypsin produces the mixture of sleep-enhancing peptides known as CTH. Within this mixture, a specific peptide known as α-casozepine (α-CZP) has been identified that could be responsible for some of these effects. Lin Zheng, Mouming Zhao and colleagues wondered if they could find other, perhaps more powerful, sleep-enhancing peptides in CTH.
The researchers first compared the effects of CTH and α-CZP in mouse sleep tests, finding that CTH showed better sleep-enhancing properties than α-CZP alone. This result suggested that other sleep-promoting peptides besides α-CZP exist in CTH. The team then used mass spectrometry to identify bioactive peptides released from CTH during simulated gastric digestion, and they virtually screened these peptides for binding to the GABA receptor and for the ability to cross the blood-brain barrier. When the strongest candidates were tested in mice, the best one (called YPVEPF) increased the number of mice that fell asleep quickly by about 25% and the sleep duration by more than 400% compared to a control group. In addition to this promising peptide, others in CTH should be explored that might enhance sleep through other pathways, the researchers say.
The authors acknowledge funding from the National Natural Science Foundation of China, the Guangdong Provincial Key R&D Program, the Shandong Provincial Key R&D Program, and the Specific Fund Program for Basic and Applied Basic Research of Guangdong Province.
The American Chemical Society (ACS) is a nonprofit organization chartered by the U.S. Congress. ACS’ mission is to advance the broader chemistry enterprise and its practitioners for the benefit of Earth and all its people. The Society is a global leader in promoting excellence in science education and providing access to chemistry-related information and research through its multiple research solutions, peer-reviewed journals, scientific conferences, eBooks and weekly news periodical Chemical & Engineering News. ACS journals are among the most cited, most trusted and most read within the scientific literature; however, ACS itself does not conduct chemical research. As a leader in scientific information solutions, its CAS division partners with global innovators to accelerate breakthroughs by curating, connecting and analyzing the world’s scientific knowledge. ACS’ main offices are in Washington, D.C., and Columbus, Ohio.
To automatically receive press releases from the American Chemical Society, contact email@example.com.
Note: ACS does not conduct research, but publishes and publicizes peer-reviewed scientific studies.