Our Halloween 2012 series continues with the highly fluorescent dye Lucifer yellow. The dye’s fluorescence under UV light makes it ideal for visualizing living and fixed cells. The lithium salt is the most water-soluble, but ammonium and potassium salts are also used. In 1978, W. W. Stewart reported that staining with Lucifer yellow reveals functional connections between cells, a process he called “dye coupling”.
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